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ultrasonic can improve saccharification ratio

Conventional hydrolysis of lignocellulose saccharification method: take pretreated lignocellulosic feedstock 10 g solid in water at a certain ratio of deionized water was added to adjust the pH value of 6 fiber raw materials according to the amount of 1% (w / w) was added luciferase, hydrolysis of a certain time after termination of the reaction was cooled to room temperature. Obtained by centrifugal separation the hydrolysis residues and sugar, reducing sugar determination DNS colorimetric sugar. Ultrasonic enzymatic reaction: with a conventional hydrolysis under the same temperature and pH conditions, the ultrasonic frequency 20kH z, the ultrasonic action time and the intermittent time for 1b2, hydrolysis of a certain period of time after termination of the reaction was cooled to room temperature. Obtained by centrifugal separation the hydrolysis residues and sugar, reducing sugar determination DNS colorimetric sugar.

The influence of ultrasound on the role of alkali pretreatment conditions using ultrasound with the alkaline hydrolysis process can reduce treatment intensity, reducing the reaction time and the amount of alkali. Was found in the comparative experiments alkali concentration needed to achieve the same rate of decomposition and hydrolysis reaction time are reduced. Ultrasonic preconditioning effect of alkaline hydrolysis conditions Table 1 Effect of a single alkaline solution the ULTRASONIC TO THE CONDITION of alkali Pretreatment pretreatment process raw material decomposition% alkali concentration% Reaction time / h reaction temperature e 24.1 8 525 ultrasound auxiliary alkaline hydrolysis 25.4 5 1 25 Ultrasonic cavitation to enhance the mass transfer, to increase the solubility of the alkali solution. Almost the same rate of decomposition of raw materials, ultrasound-assisted pretreatment required alkali concentration decreased from 8% to 5%, while the hydrolysis time from the 5 h reduced to 1 h, This article uses ultrasound-assisted alkali pretreatment process .

The ultrasonic assisted alkaline pretreatment with a single alkaline hydrolysis pretreatment compares the influence of the respective components of the raw material compared ultrasound-assisted alkaline hydrolysis pretreatment and a single alkaline hydrolysis pretreatment under the same conditions, the removal rate increased 23.3% lignin , hemicellulose degradation rate increased 12.1% alkali pretreatment advantage lies in the effective removal of lignin the micro vibration ultrasonic cavitation effect can not only further improve the removal rate of lignin and hemicellulose The degradation rate also improved significantly. The hemicellulose dissolved part of the fiber so that the pretreated feedstock prime can be improved, enzymatic saccharification rate increases. Scanning electron microscopy (SEM) analysis showed that untreated (a), a single ultrasonic treatment (b), corn straw pellet after alkaline hydrolysis (c) the apparent morphology. There is a great difference between the degree of decomposition of the raw material particles, (a) for the dense stiffness corn stalks, the energy generated by the ultrasonic oscillation (b) is not sufficient to change the surface morphology of the granular materials, (c) alkaline hydrolysis the swelling effect on the form of corn stalks raw materials have greater destructive power, fiber morphology swell, the structure becomes loose, the surface break ultrasonic assisted alkaline hydrolysis pretreatment Compare broken phenomenon, an increase in the surface area of ​​the fibers, these changes help to increase the reaction The reagents and solvents can be used and the degree of cellulose, thereby improving the response performance and solubility properties. By known ultrasound can improve the degradation rate of the semi-fiber raw materials, it can be speculated that the optimization of ultrasound-assisted alkali pretreatment to enhance the overall enzymatic saccharification rate.

Raw materials of different pretreatments SEM, infrared spectroscopy can be seen after ultrasonic treatment, hemicellulose decomposition rate increased more than doubled. This is at a disadvantage after saccharification process, for further optimization of the operation process, recovery of hemicellulose decomposition. Ultrasound-assisted alkali reaction mixture was adjusted to a weak acid, the decomposition oligosaccharides re-polymerization of cellulose dextrin precipitates out. Ultrasound-assisted pre-FT IR spectra, contrast can be found ultrasonic the lignocellulose saccharification process impact study now to the original cellulose, cellulose dextrin hydrogen absorption peak (3400 cm - 1) decreased intensity, indicating dextrin hydrogen The weakened key associating Level corresponding degree of crystallinity decreases. Its characteristic absorption bands (700, 1800 cm - 1) also have a greater weakening, Description Dextrin the low degree of polymerization. This part of the dextrin recovered directly mixed into the glycated material not only reduce the loss of the ingredients, and can effectively increase the efficiency of action of the cellulase, thereby enhancing the enzymatic saccharification ratios.

Cellulose dextrin and infrared spectra of the lignocellulose can be seen that in the starting 8 16h, and ordinary water bath in an ultrasonic bath, the the glycated rate increases with increasing time; the reaction time extended the saccharification ratios in the ultrasonic bath is significantly greater than in an ordinary bath saccharification ratios, and reaches the maximum rate of saccharification of cellulose enzyme at 48h, at 47.6%, the the cellulase catalytic effect than conventional enzymatic improve approximately 70% of its mechanism of action may be: ultrasonic direct effect on the substrate to produce a partially degraded, thereby enabling the enzymatic reaction easier; micro ultrasonic acoustic streaming certain degree of impact on the substrate and enzyme molecules, so that increase both the number of contacts, thus contributing to the increase in the reaction rate, the product may be released faster in this micro-acoustic streaming under; stable cavitation to produce a certain amount of energy, and passed to the reaction system, thereby improving the substrate into the enzyme and the product into the solute mass transfer diffusion process; ultrasonic action may change the conformation of the enzyme molecule, its spatial structure is more suitable to play a catalytic function and substrate binding.

Comparison of two pretreatment technology, ultrasonic supporting role to reduce the the alkali pretreatment reaction time and amount of alkali lignin removal rate. Through process improvements to achieve the comprehensive utilization of raw materials and reduce environmental pollution. The ultrasonic conditions used in this experiment 80W, 20 KH z, some low frequency ultrasound, it did not change the apparent form of raw materials. The application of ultrasonic in the enzymatic process, can improve the saccharification ratios. Ultrasound-assisted enzymatic hydrolysis, saccharification ratios in the present study increased by about 70% than the conventional hydrolysis.

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