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Ultrasonic extraction method can improve the yield of flavonoids experiments

Ultrasonic extraction of total flavonoids from persimmon leaves. Process: persimmon leaves crushed y y the ethanol soak y ultrasonic processing y centrifugal y to set the volume y determination. Single factor experiments. An ethanol concentration of ultrasonic extraction. Accurately weigh take persimmon leaf meal 2.0g, 6, solid-liquid ratio 1B25, were added to 40%, 50%, 60%, 70%, 80%, 90% ethanol, soaked 1 h 30 min ultrasonic extraction extract was centrifuged (4 000 r / min) 10 min, washed residue with a small amount of 70% ethanol, centrifuged 5 min. the combined centrifugal liquid buy 100 ml volumetric flask, add ethanol solution to volume, 5 ml of extract / 1 2.20 color reaction, the absorbance was measured at 510 nm. o solid-liquid ratio of ultrasonic extraction. Accurately weighed persimmon leaves meal. 000 g, 4, by solid-liquid ratio 1B10, 1B15, 1B20, 1B25 were added to 70% ethanol, the following operations with 1.? Extraction time of ultrasonic extraction. Accurately weigh take persimmon leaf meal. 000 g, 4, solid-liquid ratio 1B25, 15,30,45,60 min extraction time by choice after 1 h of immersion ultrasonic extraction, the following operations with orthogonal experiment . Under the single factor above experimental results, the experimental greater impact on the three factors each set 3 level L 9 (3 3) orthogonal experiment to determine the optimum extraction conditions of persimmon leaf flavonoids. Conventional reflux extraction of total flavonoids from persimmon leaves absorbance was measured value of 0.092, the calculated total flavonoids was 2.00%.

Ultrasonic extraction single factor experimental results: the concentration of ethanol ultrasonic extraction. With ethanol concentration, the the persimmon leaf extraction of total flavonoids rate also will increase; when the ethanol concentration is more than 60%, with the increase of the concentration of ethanol, persimmon leaf extraction of total flavonoids rate would decrease. This may be due with the increase of the concentration of ethanol extract flavonoids outside material extraction rate increases, thus affecting the extraction of flavonoids, resulting in decreased persimmon leaf extraction rate of total flavonoids. Solid-liquid ratio on ultrasonic extraction. Shows that the extraction rate of total flavonoids from persimmon leaves with the material-liquid ratio increases rapidly with increasing; solid-liquid ratio reaches 1B20, total flavonoids extraction rate of increase has slowed down significantly.

Extraction time on the ultrasonic extraction. Shows that the total extraction yield with increasing ultrasonic time an upward trend, but the trend is not very obvious, especially when the extraction time of 45 min, the extraction rate was almost unchanged. Solid-liquid ratio on ultrasonic extraction extraction time of ultrasonic extraction 2.3 orthogonal experiment results the orthogonal experiments show that the (2), persimmon leaf flavonoids extraction rate factors, the order of impact: B (solid-liquid ratio) > C (extraction time)> A (ethanol concentration), the solid-liquid ratio is the most important factor to affect the persimmon leaf total flavonoids extraction rate. Rate K values ​​obtained from the average mentioning of view, the extraction conditions A 3 B 3 C 3 best extraction process, that is, 60% ethanol, ultrasound 45 min, the solid-liquid ratio 1:25.

Orthogonal experiment experiment, ethanol concentration A feed liquid extraction time than B C blank yield of flavonoids M% 1 1. 443 2 1 2 1. 636 3 1 3 3. 042 4 2 1 2 3 2. 137 5 2 3 2 1. 771 6 2 3 1 2. 156 7 3 1 3 2 2. 811 8 3 2 3 2. 386 9 3 1 2. 464 K 1 2.040 2.130 2. 021 - K 2 2.021 1.925 2. 047 2. 073 - K 2.541 2.554 2 541 2 516 - R 0.520 0.529 0. 520 0 495 - 2 4 authentication experiment weighed persimmon leaf dry powder 2 000 g, in order to feed liquid was added 1B25 60% ethanol, soaked 1 h, ultrasonic extraction 45 min, the extract was centrifuged (4 000 r / min) 10 min, with a small amount of 70% ethanol dregs again centrifuged 15 min. the combined centrifugal liquid buy 100 ml volumetric flask, add ethanol solution to volume . Take 5ml extract / 1 2.20 color reaction, measured at 510 nm absorbance value OD = 172, persimmon leaf total flavonoids was 3.54%, with the orthogonal experimental results match.

Ultrasonic extraction method with the conventional reflux method by the known ultrasonic persimmon leaf total flavonoid extraction yield increased by 77% and shorten the extraction time 195 min, solid-liquid ratio (amount of extraction solvent) cut in half.

Ultrasonic extraction method compared with conventional reflux extraction extraction method time mmin solid-liquid ratio extraction rate M% conventional reflux method 240 of 1B55 2. 00 ultrasonic extraction 45 1B25 ultrasonic extraction Persimmon Flavone optimum conditions of A 3 B 3 C 3, 60% ethanol concentration, ultrasonic time 45 min, solid-liquid ratio 1B25 compared with conventional reflux method, ultrasonic extraction method can improve the persimmon leaf total flavonoids yield of 77% and significantly shorten the extraction time, reduce the extraction solvent dose. Therefore, ultrasonic extraction method has the obvious advantage of high extraction efficiency, and low cost.


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